|
KMID : 0357419950250020199
|
|
Korean journal of Virology
1995 Volume.25 No. 2 p.199 ~ p.206
|
|
|
The Rapid Diagnosis of Japanese Encephalitis Virus Isolated in Korea by using Dot Enzyme Immunoassay and RT-PCR.
|
|
조해월
김정림/반성자/남재환/정연준/김은정/이유정/최승은/원은하
|
|
|
Abstract
|
|
|
To the developement of the rapid viral diagnosis for Japanes Encephalitis (JE), we established the dot enzyme immunoassay (DEIA) and RT-PCR to detect an antigen and antibody against JE virus. For detection of JE antibody in swine serum using the
DEIA
method, we confirmed 100% of the sensitivity and the specificity comparison to the ELISA and HI method. beside, using three method: SDS-Proteinase K-phenol method, RNA TackTM resin Kit (Biotecx) and Guanidine thiocyanate Kit (Promega) for
isolation
RNA
of JE virus. Among these method, RNA TackTM resin Kit was detected to JE virus even 10-9dilution level which HA titer was 1:320 and this method is the most rapid isolation of RNA. The sensitivity of RT-PCR was found to be at least 10E2 to 10E7
better,
as compared to that of HA test. With the results, DEIA and RT-PCR is rapid diagnosis method to detection for JE virus and it could be applied to the other viral disease for rapid viral diagnosis.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|